Iodine in PDB 1f09: Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides

The structure of Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides, PDB code: 1f09 was solved by R.M.Wachter, D.Yarbrough, K.Kallio, S.J.Remington, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.10 / 2.14
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.197, 62.818, 68.667, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Iodine atom in the Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides (pdb code 1f09). This binding sites where shown within 5.0 Angstroms radius around Iodine atom.
In total 2 binding sites of Iodine where determined in the Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides, PDB code: 1f09:
Jump to Iodine binding site number: 1; 2;

Iodine binding site 1 out of 2 in the Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides


Mono view


Stereo pair view

A full contact list of Iodine with other atoms in the I binding site number 1 of Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides within 5.0Å range: probe atom residue distance (Å) B Occ A:I251 b:39.1 occ:0.41 O A:HOH312 3.3 33.9 1.0 CD1 A:TYR143 3.8 31.2 1.0 O A:LEU141 4.0 34.1 1.0 CD A:PRO58 4.0 29.9 1.0 O A:HOH388 4.0 43.5 1.0 O A:HOH389 4.0 39.6 1.0 CA A:TYR143 4.1 25.8 1.0 CB A:TYR143 4.2 29.5 1.0 N A:TYR143 4.2 19.9 1.0 CB A:PRO56 4.3 25.7 1.0 CG A:TYR143 4.5 30.4 1.0 N A:TRP57 4.5 19.5 1.0 C A:GLU142 4.5 33.2 1.0 CG A:PRO58 4.5 30.2 1.0 O A:GLU142 4.6 28.2 1.0 CA A:PRO56 4.6 28.0 1.0 CE1 A:TYR143 4.8 25.1 1.0 CB A:TRP57 4.8 26.2 1.0 C A:LEU141 4.9 30.2 1.0 C A:PRO56 4.9 31.1 1.0

Iodine binding site 2 out of 2 in the Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides


Mono view


Stereo pair view

A full contact list of Iodine with other atoms in the I binding site number 2 of Crystal Structure of the Green Fluorescent Protein (Gfp) Variant Yfp-H148Q with Two Bound Iodides within 5.0Å range: probe atom residue distance (Å) B Occ A:I254 b:30.2 occ:0.60 NE2 A:GLN69 3.2 17.2 1.0 OH A:TYR203 3.3 42.3 1.0 OE1 A:GLN183 3.8 40.4 1.0 CG A:GLN69 3.8 42.9 1.0 CD A:GLN69 3.9 66.6 1.0 NH2 A:ARG96 4.1 23.5 1.0 CE1 A:TYR203 4.1 37.3 1.0 CZ A:TYR203 4.1 37.6 1.0 CG1 A:VAL150 4.2 25.1 1.0 O2 A:CR266 4.2 29.4 1.0 CD1 A:LEU201 4.3 31.1 1.0 CB A:VAL150 4.3 25.2 1.0 CD1 A:ILE152 4.4 23.2 1.0 CB2 A:CR266 4.4 38.0 1.0 CD2 A:CR266 4.5 39.6 1.0 CG2 A:VAL150 4.5 19.0 1.0 CD A:GLN183 4.6 62.1 1.0 CG1 A:VAL163 4.8 43.6 1.0 NE2 A:GLN183 4.8 72.1 1.0 CE2 A:PHE165 4.8 34.6 1.0 CG2 A:CR266 4.9 35.6 1.0 CG2 A:VAL163 5.0 39.1 1.0 CZ A:PHE165 5.0 29.3 1.0

R.M.Wachter, D.Yarbrough, K.Kallio, S.J.Remington. Crystallographic and Energetic Analysis of Binding of Selected Anions to the Yellow Variants of Green Fluorescent Protein. J.Mol.Biol. V. 301 157 2000.
ISSN: ISSN 0022-2836
PubMed: 10926499
DOI: 10.1006/JMBI.2000.3905