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Iodine in PDB 6dol: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2)

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2)

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2):
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2), PDB code: 6dol was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.06 / 1.43
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.801, 37.113, 62.105, 90.00, 96.44, 90.00
R / Rfree (%) 15.1 / 18.5

Other elements in 6dol:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2) also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Potassium (K) 1 atom

Iodine Binding Sites:

The binding sites of Iodine atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2) (pdb code 6dol). This binding sites where shown within 5.0 Angstroms radius around Iodine atom.
In total 2 binding sites of Iodine where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2), PDB code: 6dol:
Jump to Iodine binding site number: 1; 2;

Iodine binding site 1 out of 2 in 6dol

Go back to Iodine Binding Sites List in 6dol
Iodine binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2)


Mono view


Stereo pair view

A full contact list of Iodine with other atoms in the I binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:I204

b:37.9
occ:0.35
HH A:TYR83 2.4 33.9 1.0
HD22 A:LEU161 3.0 38.4 1.0
HD13 A:LEU161 3.2 35.7 1.0
OH A:TYR83 3.2 28.2 1.0
HE2 A:TYR83 3.3 32.2 1.0
HD22 A:LEU111 3.3 23.9 1.0
HB3 A:LEU161 3.4 27.1 1.0
O A:HOH420 3.4 44.6 1.0
HD13 A:LEU111 3.4 26.8 1.0
HD3 A:ARG97 3.4 34.9 1.0
HB3 A:LEU111 3.5 23.0 1.0
O A:HOH361 3.8 25.3 1.0
CD2 A:LEU161 3.9 32.0 1.0
HH11 A:ARG97 3.9 48.9 1.0
CE2 A:TYR83 4.0 26.9 1.0
CD1 A:LEU161 4.0 29.8 1.0
CZ A:TYR83 4.1 23.4 1.0
CB A:LEU161 4.2 22.6 1.0
CD2 A:LEU111 4.2 19.9 1.0
CG A:LEU161 4.3 26.3 1.0
HG2 A:ARG97 4.3 28.8 1.0
CD1 A:LEU111 4.3 22.4 1.0
HD21 A:LEU161 4.4 38.4 1.0
CB A:LEU111 4.4 19.2 1.0
CD A:ARG97 4.4 29.1 1.0
NH1 A:ARG97 4.4 40.7 1.0
O A:HOH338 4.5 33.0 1.0
HD11 A:LEU161 4.5 35.7 1.0
HD23 A:LEU161 4.5 38.4 1.0
CG A:LEU111 4.5 18.7 1.0
HB2 A:HIS115 4.7 22.7 1.0
HD12 A:LEU161 4.7 35.7 1.0
HB2 A:LEU161 4.7 27.1 1.0
HD21 A:LEU111 4.7 23.9 1.0
HD11 A:ILE100 4.8 33.5 1.0
HH12 A:ARG97 4.8 48.9 1.0
HD23 A:LEU111 4.8 23.9 1.0
O A:HOH410 4.8 27.9 1.0
HD11 A:LEU111 4.8 26.8 1.0
CG A:ARG97 4.8 24.0 1.0
HB2 A:LEU111 4.9 23.0 1.0
HD12 A:LEU111 4.9 26.8 1.0
HB2 A:ARG97 4.9 27.7 1.0
HD2 A:ARG97 5.0 34.9 1.0
NE A:ARG97 5.0 29.9 1.0

Iodine binding site 2 out of 2 in 6dol

Go back to Iodine Binding Sites List in 6dol
Iodine binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2)


Mono view


Stereo pair view

A full contact list of Iodine with other atoms in the I binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 25 Mm K+ For 120 S at 21 C (Dataset 2) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:I205

b:42.9
occ:0.25
O A:HOH463 2.9 44.1 1.0
HG2 A:PRO177 2.9 27.3 1.0
O A:HOH460 3.0 36.3 1.0
HD11 A:LEU179 3.1 29.1 1.0
HG3 A:PRO128 3.2 29.0 1.0
HB3 A:PRO128 3.3 23.5 1.0
HB2 A:PRO177 3.4 26.7 1.0
HD21 A:LEU179 3.6 31.8 1.0
CD1 A:ILE64 3.7 42.9 1.0
CG A:PRO177 3.7 22.7 1.0
HB3 A:PRO177 3.8 26.7 1.0
CB A:PRO177 3.8 22.2 1.0
CG A:PRO128 4.0 24.2 1.0
CB A:PRO128 4.1 19.6 1.0
CD1 A:LEU179 4.1 24.2 1.0
HG3 A:PRO177 4.2 27.3 1.0
O A:HOH465 4.3 55.1 1.0
HD13 A:LEU179 4.4 29.1 1.0
HD3 A:PRO128 4.4 23.9 1.0
HB2 A:PRO128 4.4 23.5 1.0
CD2 A:LEU179 4.4 26.5 1.0
HD2 A:PRO177 4.6 24.2 1.0
HD12 A:LEU179 4.6 29.1 1.0
CG A:LEU179 4.7 22.7 1.0
HD22 A:LEU179 4.7 31.8 1.0
HG2 A:PRO128 4.7 29.0 1.0
HG A:LEU179 4.7 27.3 1.0
CD A:PRO128 4.8 19.9 1.0
CD A:PRO177 4.8 20.2 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sun Aug 11 22:56:10 2024

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